Measurement
M_betB

Part:BBa_K5088504:Design

Designed by: Dascha Khalfine   Group: iGEM24_Marburg   (2024-07-31)


Measurement construct - betB promoter+5'UTR from T. kok-saghyz


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 958
    Illegal XbaI site found at 954
    Illegal XbaI site found at 3500
    Illegal PstI site found at 3927
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 958
    Illegal PstI site found at 3927
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 958
    Illegal BglII site found at 3503
    Illegal XhoI site found at 1588
    Illegal XhoI site found at 2747
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 958
    Illegal XbaI site found at 954
    Illegal XbaI site found at 3500
    Illegal PstI site found at 3927
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 958
    Illegal XbaI site found at 954
    Illegal XbaI site found at 3500
    Illegal PstI site found at 3927
    Illegal NgoMIV site found at 2975
    Illegal AgeI site found at 402
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 3189


Design Notes

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Source

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References